HLA-I immunopeptidome profiling of human cells infected with high-containment enveloped viruses.

ABSTRACT: Immunopeptidome profiling of infected cells is a powerful technique for detecting viral peptides that are naturally processed and loaded onto class I human leukocyte antigens (HLAs-I). Here, we provide a protocol for preparing samples for immunopeptidome profiling that can inactivate enveloped viruses while still preserving the integrity of the HLA-I complex. We detail steps for lysate preparation of infected cells followed by HLA-I immunoprecipitation and virus inactivation. We further describe peptide purification for mass spectrometry outside a high-containment facility. For complete details on the use and execution of this protocol, please refer to Weingarten-Gabbay et al. (2021).¹.

SUBMITTER: Weingarten-Gabbay S 

PROVIDER: S-EPMC9731565 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature