Correlation of cell mechanics with the resistance to malignant transformation in naked mole rat fibroblasts.

ABSTRACT: Naked mole rats (NMRs) demonstrate exceptional longevity and resistance to cancer. Using a biochemical approach, it was previously shown that the treatment of mouse fibroblast cells with RasV12 oncogene and SV40 Large T antigen (viral oncoprotein) led to malignant transformations of cells. In contrast, NMR fibroblasts were resistant to malignant transformations upon this treatment. Here we demonstrate that atomic force microscopy (AFM) can provide information which is in agreement with the above finding, and further, adds unique information about the physical properties of cells that is impossible to obtain by other existing techniques. AFM indentation data were collected from individual cells and subsequently processed through the brush model to obtain information about the mechanics of t

SUBMITTER: Makarova N 

PROVIDER: S-EPMC9731726 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature