An alkaline phosphatase-induced immunosensor for SARS-CoV-2 N protein and cardiac troponin I based on the in situ fluorogenic self-assembly between N-heterocyclic boronic acids and alizarin red S.

ABSTRACT: Alkaline phosphatase (ALP)-induced in situ fluorescent immunosensor is less investigated and reported. Herein, a high-performance ALP-labeled in situ fluorescent immunoassay platform was constructed. The developed platform was based on a fluorogenic self-assembly reaction between pyridineboronic acid (PyB(OH)₂) and alizarin red S (ARS). We first used density functional theory (DFT) to theoretically calculate the changes of Gibbs free energy of the used chemicals before and after the combination and simulated the electrostatic potential on its' surfaces. The free ARS and PyB(OH)₂ exist alone, neither emits no fluorescence. However, the ARS/PyB(OH)₂ complex emits strong fluorescence, which could be effectively quenched by PPi based on the stronger affinity be

SUBMITTER: Geng F 

PROVIDER: S-EPMC9731814 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature