Isolated murine skeletal muscles utilize pyruvate over glucose for oxidation.

ABSTRACT:

Introduction

Fuel sources for skeletal muscle tissue include carbohydrates and fatty acids, and utilization depends upon fiber type, workload, and substrate availability. The use of isotopically labeled substrate tracers combined with nuclear magnetic resonance (NMR) enables a deeper examination of not only utilization of substrates by a given tissue, but also their contribution to tricarboxylic acid (TCA) cycle intermediates.

Objectives

The goal of this study was to determine the differential utilization of substrates in isolated murine skeletal muscle, and to evaluate how isopotomer anlaysis provided insight into skeletal muscle metabolism.

Methods

Isolated C57BL/6 mouse hind limb muscles were incubated in oxygenated solution containing uniformly labeled ¹³

SUBMITTER: Khattri RB 

PROVIDER: S-EPMC9732067 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature