Single-cell CRISPR immune screens reveal immunological roles of tumor intrinsic factors.

ABSTRACT: Genetic screens are widely exploited to develop novel therapeutic approaches for cancer treatment. With recent advances in single-cell technology, single-cell CRISPR screen (scCRISPR) platforms provide opportunities for target validation and mechanistic studies in a high-throughput manner. Here, we aim to establish scCRISPR platforms which are suitable for immune-related screens involving multiple cell types. We integrated two scCRISPR platforms, namely Perturb-seq and CROP-seq, with both in vitro and in vivo immune screens. By leveraging previously generated resources, we optimized experimental conditions and data analysis pipelines to achieve better consistency between results from high-throughput and individual validations. Furthermore, we evaluated the performance of scCR

SUBMITTER: Hou J 

PROVIDER: S-EPMC9732527 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature