Ontology highlight
ABSTRACT:
SUBMITTER: Williams JS
PROVIDER: S-EPMC9732815 | biostudies-literature | 2022 Oct
REPOSITORIES: biostudies-literature
Williams Jessica S JS Wojtaszek Jessica L JL Appel Denise C DC Krahn Juno J Wallace Bret D BD Walsh Evan E Kunkel Thomas A TA Williams R Scott RS
Cell reports 20221001 1
Topoisomerase 1 (Top1) incises DNA containing ribonucleotides to generate complex DNA lesions that are resolved by APE2 (Apn2 in yeast). How Apn2 engages and processes this DNA damage is unclear. Here, we report X-ray crystal structures and biochemical analysis of Apn2-DNA complexes to demonstrate how Apn2 frays and cleaves 3' DNA termini via a wedging mechanism that facilitates 1-6 nucleotide endonucleolytic cleavages. APN2 deletion and DNA-wedge mutant Saccharomyces cerevisiae strains display ...[more]