Glioblastoma cells have increased capacity to repair radiation-induced DNA damage after migration to the olfactory bulb.

ABSTRACT:

Background

The invasive nature of GBM combined with the diversity of brain microenvironments creates the potential for a topographic heterogeneity in GBM radioresponse. Investigating the mechanisms responsible for a microenvironment-induced differential GBM response to radiation may provide insights into the molecules and processes mediating GBM radioresistance.

Methods

Using a model system in which human GBM stem-like cells implanted into the right striatum of nude mice migrate throughout the right hemisphere (RH) to the olfactory bulb (OB), the radiation-induced DNA damage response was evaluated in each location according to γH2AX and 53BP1 foci and cell cycle phase distribution as determined by flow cytometry and immunohistochemistry. RNAseq was used to compare transcript

SUBMITTER: Degorre C 

PROVIDER: S-EPMC9733339 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature