Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon.

ABSTRACT: Single-particle analysis (SPA) by cryo-electron microscopy (cryo-EM) is now a mainstream technique for high-resolution structural biology. Structure determination by SPA relies upon obtaining multiple distinct views of a macromolecular object vitrified within a thin layer of ice. Ideally, a collection of uniformly distributed random projection orientations would amount to all possible views of the object, giving rise to reconstructions characterized by isotropic directional resolution. However, in reality, many samples suffer from preferentially oriented particles adhering to the air-water interface. This leads to non-uniform angular orientation distributions in the dataset and inhomogeneous Fourier-space sampling in the reconstruction, translating into maps characterized by anisotropic re

SUBMITTER: Aiyer S 

PROVIDER: S-EPMC9733953 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature