Rapid Assessment of Neutralizing Antibodies Using Influenza Viruses with a Luciferase Reporter.

ABSTRACT: Influenza viruses cause acute respiratory infections, especially in the autumn-winter period. They are characterized by a high mutation frequency and cause annual seasonal epidemics. The detection of antibodies that neutralize the virus is an important criterion in the assessment of population immunity and the influenza vaccine effectiveness. In this study, a method for determining the titer of virus-neutralizing antibodies in blood serum has been developed. A new test called the luciferase neutralization assay uses a bioluminescent signal for detection. The assay is based on engineered influenza reporter viruses with various surface antigens and a nanoluciferase reporter protein in the NS1 reading frame. Using the developed method, we studied paired sera of volunteers obtained before and

SUBMITTER: Sergeeva MV 

PROVIDER: S-EPMC9734512 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature