Close relatives of MERS-CoV in bats use ACE2 as their functional receptors.

ABSTRACT: Middle East respiratory syndrome coronavirus (MERS-CoV) and several bat coronaviruses use dipeptidyl peptidase-4 (DPP4) as an entry receptor^1-4. However, the receptor for NeoCoV-the closest known MERS-CoV relative found in bats-remains unclear⁵. Here, using a pseudotype virus entry assay, we found that NeoCoV and its close relative, PDF-2180, can efficiently bind to and use specific bat angiotensin-converting enzyme 2 (ACE2) orthologues and, less favourably, human ACE2 as entry receptors through their receptor-binding domains (RBDs) on the spike (S) proteins. Cryo-electron microscopy analysis revealed an RBD-ACE2 binding interface involving protein-glycan interactions, distinct from those of other known ACE2-using coronaviruses. We identified residues 337-342 of human

SUBMITTER: Xiong Q 

PROVIDER: S-EPMC9734910 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature