Inhibitory Effects of Nobiletin on Voltage-Gated Na⁺ Channel in Rat Ventricular Myocytes Based on Electrophysiological Analysis and Molecular Docking Method.

ABSTRACT: Nobiletin (NOB) has attracted much attention owing to its outstanding bioactivities. This study aimed to investigate its anti-arrhythmic effect through electrophysiological and molecular docking studies. We assessed the anti-arrhythmic effects of NOB using aconitine-induced ventricular arrhythmia in a rat model and the electrophysiological effects of NOB on rat cardiomyocytes utilizing whole-cell patch-clamp techniques. Moreover, we investigated the binding characters of NOB with rNav1.5, rNav1.5/QQQ, and hNa_V1.5 via docking analysis, comparing them with amiodarone and aconitine. NOB pretreatment delayed susceptibility to ventricular premature and ventricular tachycardia and decreased the incidence of fatal ventricular fibrillation. Whole-cell patch-clamp assays demonstrated tha

SUBMITTER: Gu Y 

PROVIDER: S-EPMC9736168 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature