1,2-Hydrogenation and Transhydrogenation Catalyzed by 3-Ketosteroid Δ¹-Dehydrogenase from Sterolibacterium denitrificans-Kinetics, Isotope Labelling and QM:MM Modelling Studies.

ABSTRACT: Bacteria and fungi that are able to metabolize steroids express 3-ketosteroid-Δ¹-dehydrogenases (KstDs). KstDs such as AcmB form Sterolibacterium denitrificans Chol-1 catalyze the enantioselective 1α,2β-dehydrogenation of steroids to their desaturated analogues, e.g., the formation of 1,4-androstadiene-3,17-dione (ADD) from 4-androsten-3,17-dione (AD). The reaction catalyzed by KstD can be reversed if the appropriate electron donor, such as benzyl viologen radical cation, is present. Furthermore, KstDs can also catalyze transhydrogenation, which is the transfer of H atoms between 3-ketosteroids and 1-dehydrosteroids. In this paper, we showed that AcmB exhibits lower pH optima for hydrogenation and dehydrogenation by 3.5-4 pH units than those observed for KstD from Noca

SUBMITTER: Wojtkiewicz AM 

PROVIDER: S-EPMC9736390 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature