Genomewide m⁶A Mapping Uncovers Dynamic Changes in the m⁶A Epitranscriptome of Cisplatin-Treated Apoptotic HeLa Cells.

ABSTRACT: Cisplatin (CP), which is a conventional cancer chemotherapeutic drug, induces apoptosis by modulating a diverse array of gene regulatory mechanisms. However, cisplatin-mediated changes in the m⁶A methylome are unknown. We employed an m⁶A miCLIP-seq approach to investigate the effect of m⁶A methylation marks under cisplatin-mediated apoptotic conditions on HeLa cells. Our high-resolution approach revealed numerous m⁶A marks on 972 target mRNAs with an enrichment on 132 apoptotic mRNAs. We tracked the fate of differentially methylated candidate mRNAs under METTL3 knockdown and cisplatin treatment conditions. Polysome profile analyses revealed perturbations in the translational efficiency of PMAIP1 and PHLDA1 transcripts. Congru

SUBMITTER: Alasar AA 

PROVIDER: S-EPMC9738315 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature