Dataset Information

The Biodegradation of Indigo Carmine by Bacillus safensis HL3 Spore and Toxicity Analysis of the Degradation Products.

ABSTRACT: The aims of this article were to investigate Bacillus safensis HL3 spore for its capacity to degrade and detoxify indigo carmine and to provide an effective biological agent for the treatment of isatin dye wastewater. Bacillus safensis HL3 spore was found to decolorize indigo carmine by 97% in the presence of acetosyringone within 2 h. Significantly increased activities of spore laccase, intracellular tyrosinase, and lignin peroxidase upon exposure to indigo carmine were observed. The results of RT-qPCR also showed that the expression of laccase gene was significantly increased. The spore has the ability to degrade indigo carmine through oxidization. Furthermore, the pathway by which indigo carmine is degraded was investigated using liquid chromatography-mass spectrometry analysis to identify the biodegradation products. A detailed pathway of indigo carmine degradation by bacterial spores was proposed for the first time. Toxicity tests indicated that the biodegradation products of indigo carmine are non-toxic to Nicotiana tabacum seeds and are less hazardous to human erythrocytes than the original dye. Indigo carmine is a typical recalcitrant dye and severely jeopardizes human health. The results demonstrate the utility of the spore from Bacillus safensis HL3 for the degradation of indigo carmine and simultaneous reduction of its toxicity.

SUBMITTER: Wang C

PROVIDER: S-EPMC9738463 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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The Biodegradation of Indigo Carmine by Bacillus safensis HL3 Spore and Toxicity Analysis of the Degradation Products.

Wang Chunlei C Wang Sijia S Zhang Jieru J Jiang Shumin S Cui Daizong D Sun Haiqiong H Liu Chengwei C Li Lili L Zhao Min M

Molecules (Basel, Switzerland) 20221204 23

The aims of this article were to investigate Bacillus safensis HL3 spore for its capacity to degrade and detoxify indigo carmine and to provide an effective biological agent for the treatment of isatin dye wastewater. Bacillus safensis HL3 spore was found to decolorize indigo carmine by 97% in the presence of acetosyringone within 2 h. Significantly increased activities of spore laccase, intracellular tyrosinase, and lignin peroxidase upon exposure to indigo carmine were observed. ...[more]

PMID: 36500632

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Project description:Biodegradation of pollutants often results in incomplete mineralization and formation of degradation products with unknown chemical and toxicological characteristics. Ultraviolet (UV) irradiation, a common technology used in water and wastewater treatment, may help reduce aqueous concentrations of degradation products produced during biological treatment and their associated hazards. Combined biological and UV transformations may be important in natural systems as well. We investigated the effects of UV irradiation (254 nm) on dibenzothiophene (DBT), a sulfur-containing polyaromatic hydrocarbon, in artificial seawater, and its effects on biodegradation products produced from mixed-community microbial transformations of DBT, including DBT sulfone, DBT sulfoxide, hydroxylated and carboxylated benzothiophenes, thiosalicylic acid, and others. Toxicity of solutions after UV exposure was monitored using bioluminescent bacteria (Vibrio fischeri) and by evaluating cardiac deformities in Fundulus heteroclitus embryos. The highest UV fluence reduced DBT concentration by 28% when DBT was present as the sole organic solute. In postbiodegradation solution, the same fluence reduced the initial concentration of DBT by 81%, and 11 DBT biodegradation products to trace levels. Regardless of UV fluence, DBT by itself produced minimal effects in Fundulus embryos but was moderately toxic to V. fischeri. Postbiodegradation solutions were highly toxic to both test organisms. The highest UV fluence slightly reduced toxicity of postbiodegradation solution to V. fischeri but exacerbated cardiac deformities in Fundulus embryos. Toxicity could not be attributed to specific products and was likely a result of mixture effects. These results emphasize that toxicity can increase during remediation and that multiple assays may be necessary for evaluation. The novel approach of combined biodegradation/UV treatment is promising, although further research is needed to reduce toxicity in the case of DBT.

Dataset Information

The Biodegradation of Indigo Carmine by Bacillus safensis HL3 Spore and Toxicity Analysis of the Degradation Products.

Publications

The Biodegradation of Indigo Carmine by <i>Bacillus safensis</i> HL3 Spore and Toxicity Analysis of the Degradation Products.

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