Oxygen Gradient Induced in Microfluidic Chips Can Be Used as a Model for Liver Zonation.

ABSTRACT: Availability of oxygen plays an important role in tissue organization and cell-type specific metabolism. It is, however, difficult to analyze hypoxia-related adaptations in vitro because of inherent limitations of experimental model systems. In this study, we establish a microfluidic tissue culture protocol to generate hypoxic gradients in vitro, mimicking the conditions found in the liver acinus. To accomplish this, four microfluidic chips, each containing two chambers, were serially connected to obtain eight interconnected chambers. HepG2 hepatocytes were uniformly seeded in each chamber and cultivated under a constant media flow of 50 µL/h for 72 h. HepG2 oxygen consumption under flowing media conditions established a normoxia to hypoxia gradient within the chambers, which was confirmed

SUBMITTER: Ghafoory S 

PROVIDER: S-EPMC9738923 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature