Knockdown of YY1 Inhibits XIST Expression and Enhances Cloned Pig Embryo Development.

ABSTRACT: The technique of cloning has wide applications in animal husbandry and human biomedicine. However, the very low developmental efficiency of cloned embryos limits the application of cloning. Ectopic XIST-expression-induced abnormal X chromosome inactivation (XCI) is a primary cause of the low developmental competence of cloned mouse and pig embryos. Knockout or knockdown of XIST improves cloning efficiency in both pigs and mice. The transcription factor Yin yang 1(YY1) plays a critical role in XCI by triggering the transcription of X-inactive specific transcript (XIST) and facilitating the localization of XIST RNA on the X chromosome. This study aimed to investigate whether RNA interference to suppress the expression of YY1 can inhibit erroneous XIST expre

SUBMITTER: Dong Y 

PROVIDER: S-EPMC9739934 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature