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Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes.

ABSTRACT: Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner. In this study, we designed and constructed open-form oligomers of a bimolecular form of an RNase P ribozyme. The ribozyme oligomers were analyzed biochemically and by atomic force microscopy (AFM).

SUBMITTER: Siddika MA

PROVIDER: S-EPMC9740620 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes.

Siddika Mst Ayesha MA Yamada Takahiro T Aoyama Risako R Hidaka Kumi K Sugiyama Hiroshi H Endo Masayuki M Matsumura Shigeyoshi S Ikawa Yoshiya Y

Molecules (Basel, Switzerland) 20221128 23

Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner. In this study, we designed and constructed open-form oligomers of a bimolecular form of an RNase P ribozyme. The ribozyme oligomers were analyzed biochemically and by atomic force microscopy (AFM). ...[more]

PMID: 36500390

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