HPV16-E6/E7 Oncogene Mutation and p53 Expression among Indonesian Women with Cervical Cancer.

ABSTRACT:

Objective

To characterize HPV16 E6/E7 mutation and its association with p53 expression among Indonesian women with cervical cancer.

Methods

This is a cross-sectional study involving 31 Indonesian women with pathologically proven cervical cancer and HPV16 infection. Data about the clinical characteristics of the study population were obtained from the medical records. Biopsy specimen of the cervical cancer mass from each study participant was obtained for DNA isolation. The ORFs of E6 and E7 genes were amplified using specific primer designed according to K02718/HPV16R gene sequence obtained from GenBank. Sequencing was performed using software program MEGA10. HPV16 E6 and E7 prototype sequences for nucleotide alignment (HPv16. P, GenBank Access code: NC_001526) was selected

SUBMITTER: Mahendra INB 

PROVIDER: S-EPMC9741891 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature