High Spatial Resolution MALDI Imaging Mass Spectrometry of Fresh-Frozen Bone.

ABSTRACT: Bone and bone marrow are vital to mammalian structure, movement, and immunity. These tissues are also commonly subjected to molecular alterations giving rise to debilitating diseases like rheumatoid arthritis and osteomyelitis. Technologies such as matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) facilitate the discovery of spatially resolved chemical information in biological tissue samples to help elucidate the complex molecular processes underlying pathology. Traditionally, preparation of osseous tissue for MALDI IMS has been difficult due to its mineralized composition and heterogeneous morphology, and compensation for these challenges with decalcification and fixation protocols can remove or delocalize molecular species. Here, sample preparation meth

SUBMITTER: Good CJ 

PROVIDER: S-EPMC9741954 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature