Human IgE mAbs identify major antigens of parasitic worm infection.

ABSTRACT:

Background

Much of our understanding of the targets of IgE comes from studies of allergy, though little is known about the natural immunogenic targets seen after parasitic worm infections.

Objective

We used human monoclonal antibodies (mAbs) for an unbiased and comprehensive characterization of the immunodominant antigens targeted by IgE in conditions like allergy or helminth infection that are associated with elevated levels of IgE.

Methods

Using human hybridoma technology to immortalize IgE encoding B-cells from peripheral blood of subjects with filarial infections and elevated IgE, we generated naturally occurring human IgE mAbs. B-cell cultures were screened in an unbiased manner for IgE production without regard to specificity. Isolated IgE mAbs were then tested

SUBMITTER: Hadadianpour A 

PROVIDER: S-EPMC9742163 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature