Characterization of a virulence-modifying protein of Leptospira interrogans identified by shotgun phage display.

ABSTRACT: Pathogenic species of Leptospira are etiologic agents of leptospirosis, an emerging zoonotic disease of worldwide extent and endemic in tropical regions. The growing number of identified leptospiral species sheds light to their genetic diversity and unique virulence mechanisms, many of them still remain unknown. Toxins and adhesins are important virulence factors in several pathogens, constituting promising antigens for the development of vaccines with cross-protection and long-lasting effect against leptospirosis. For this aim, we used the shotgun phage display technique to unravel new proteins with adhesive properties. A shotgun library was constructed using fragmented genomic DNA from Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 and pG8SAET phagemid vec

SUBMITTER: Lauretti-Ferreira F 

PROVIDER: S-EPMC9742253 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature