AssignSLP_GUI, a software tool exploiting AI for NMR resonance assignment of sparsely labeled proteins.

ABSTRACT: Not all proteins are amenable to uniform isotopic labeling with ¹³C and ¹⁵N, something needed for the widely used, and largely deductive, triple resonance assignment process. Among them are proteins expressed in mammalian cell culture where native glycosylation can be maintained, and proper formation of disulfide bonds facilitated. Uniform labeling in mammalian cells is prohibitively expensive, but sparse labeling with one or a few isotopically enriched amino acid types is an option for these proteins. However, assignment then relies on accessing the best match between a variety of measured NMR parameters and predictions based on 3D structure, often from X-ray crystallography. Finding this match is a challenging process that has benefitted from many computational tool

SUBMITTER: Williams RV 

PROVIDER: S-EPMC9742323 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature