Phosphatase inhibition by sodium orthovanadate displays anti-inflammatory action by suppressing AKT-IKKβ signaling in RAW264.7 cells.

ABSTRACT: Sodium orthovanadate (Na₃VO₄) is an inhibitor of phosphatases that acts as a phosphate analog and is being developed as an anti-diabetes drug. Phosphatases play important roles in inflammatory signal pathways by modulating the removal of phosphate moieties of key signaling proteins. However, the role of protein phosphatases on the inflammatory response has not been fully established. In this study, we investigated how phosphatases can control the inflammatory response using Na₃VO₄ in LPS-stimulated RAW264.7 cells and explored the molecular mechanisms by NO assay, mRNA analysis, immunoblotting analysis, kinase assay, luciferase reporter gene assay, and mutation strategy. Na₃VO₄ decreased the release of nitric oxide (NO) and s

SUBMITTER: Kim HG 

PROVIDER: S-EPMC9742970 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature