Characterization of a novel theta-type replicon of indigenous plasmid pTE15 from Lactobacillus reuteri N16.

ABSTRACT:

Background

pTE15 is a ~ 15-kb narrow-host-range indigenous plasmid from Lactobacillus reuteri N16 that does not replicate in selected Bacillus spp., Staphylococcus spp., and other Lactobacillus spp.

Methods

Combined deletion analysis the minireplicon essential of pTE15 with replicon-probe vector pUE80 (-) to confirmed sufficient for replication and from the ssDNA intermediate detection, plasmid amplification tested by chloramphenicol treatment, and replication origin sequence analysis to delineated the novel theta-type replication of pTE15.

Results

Single-stranded intermediate of pTE15 DNA was not detected in L. reuteri, indicating that this plasmid does not replicate via a rolling circle mechanism. The replicon of pTE15 did not display the structural organization typ

SUBMITTER: Chen PW 

PROVIDER: S-EPMC9743546 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature