Antibody-free measurement of cerebrospinal fluid tau phosphorylation across the Alzheimer's disease continuum.

ABSTRACT:

Background

Alzheimer's disease is characterized by an abnormal increase of phosphorylated tau (pTau) species in the CSF. It has been suggested that emergence of different pTau forms may parallel disease progression. Therefore, targeting multiple specific pTau forms may allow for a deeper understanding of disease evolution and underlying pathophysiology. Current immunoassays measure pTau epitopes separately and may capture phosphorylated tau fragments of different length depending on the non-pTau antibody used in the assay sandwich pair, which bias the measurement.

Methods

We developed the first antibody-free mass spectrometric method to simultaneously measure multiple phosphorylated epitopes in CSF tau: pT181, pS199, pS202, pT205, pT217, pT231, and pS396. The method was firs

SUBMITTER: Gobom J 

PROVIDER: S-EPMC9743664 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature