A simple and robust method for isolating and analyzing chromatin-bound RNAs in Arabidopsis.

ABSTRACT:

Background

Chromatin-bound RNAs are the primary product of transcription that undergo on-chromatin processing such as capping, splicing, and polyadenylation. These processing steps then determine the fate of RNAs. Albeit its vital importance, a simple and robust method for isolating different fractions of chromatin-bound RNAs is missing in plants.

Result

Here, we describe our updated method and the associated step-by-step protocol for chromatin-bound RNAs isolation in A. thaliana. The chromatin-bound RNAs isolation is based on the 1 M UREA wash that removes the majority of non-chromatin-associated proteins from the nucleus, as previously developed in mammalian cells. On-demand, the isolated chromatin-bound RNAs can be either used directly for gene-specific analysis or subjec

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC9743689 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature