Amplification Free Detection of SARS-CoV-2 Using Multi-Valent Binding.

ABSTRACT: We present the development of electrochemical impedance spectroscopy (EIS)-based biosensors for sensitive detection of SARS-CoV-2 RNA using multi-valent binding. By increasing the number of probe-target binding events per target molecule, multi-valent binding is a viable strategy for improving the biosensor performance. As EIS can provide sensitive and label-free measurements of nucleic acid targets during probe-target hybridization, we used multi-valent binding to build EIS biosensors for targeting SARS-CoV-2 RNA. For developing the biosensor, we explored two different approaches including probe combinations that individually bind in a single-valent fashion and the probes that bind in a multi-valent manner on their own. While we found excellent biosensor performance using probe combinatio

SUBMITTER: Roychoudhury A 

PROVIDER: S-EPMC9743695 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature