Structural and functional distinctions of co-resident microglia and monocyte-derived macrophages after retinal degeneration.

ABSTRACT:

Background

Both resident microglia and invading peripheral immune cells can respond to injury and degeneration in the central nervous system. However, after dead and dying neurons have been cleared and homeostasis is re-established, it is unknown whether resident immune cells fully resume normal functions and to what degree the peripheral immune cells take up residence.

Methods

Using flow cytometry, in vivo retinal imaging, immunohistochemistry, and single-cell mRNA sequencing, we assess resident microglia and monocyte-derived macrophages in the retina during and after the loss of photoreceptors in the Arr1^-/- mouse model of inducible degeneration.

Results

We find that photoreceptor loss results in a small, sustained increase in mononuclear phagocytes and,

SUBMITTER: Ronning KE 

PROVIDER: S-EPMC9743742 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature