Evaluation of genome and base editing tools in maize protoplasts.

ABSTRACT:

Introduction

Despite its rapid worldwide adoption as an efficient mutagenesis tool, plant genome editing remains a labor-intensive process requiring often several months of in vitro culture to obtain mutant plantlets. To avoid a waste in time and money and to test, in only a few days, the efficiency of molecular constructs or novel Cas9 variants (clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9) prior to stable transformation, rapid analysis tools are helpful.

Methods

To this end, a streamlined maize protoplast system for transient expression of CRISPR/Cas9 tools coupled to NGS (next generation sequencing) analysis and a novel bioinformatics pipeline was established.

Results and discussion

Mutation types found with high fr

SUBMITTER: Fierlej Y 

PROVIDER: S-EPMC9744195 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature