Unknown

Dataset Information

0

Advanced assessment through intact glycopeptide analysis of Infliximab's biologics and biosimilar.


ABSTRACT: Characterization of therapeutic monoclonal antibodies (mAbs) represents a major challenge for analytical sciences due to their heterogeneity associated with post-translational modifications (PTMs). The protein glycosylation requires comprehensive identification, which could influence on the mAbs' structure and their function. Here, we demonstrated high-resolution tandem mass spectrometry with an ultra-high-performance liquid chromatography for characterization and comparison between biologics and biosimilar of infliximab at an advanced level. Comparing the N- and O-glycopeptides profiles, a total of 49 and 54 glycopeptides was identified for each product of the biologics and biosimilar, respectively. We also discovered one novel N-glycosylation site at the light chain from both biopharmaceuticals and one novel O-glycopeptide at the heavy chain from only biosimilar. Site-specific glycopeptide analysis process will be a robust and useful technique for evaluating therapeutic mAbs and complex glycoprotein products.

SUBMITTER: Kim H 

PROVIDER: S-EPMC9745114 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

altmetric image

Publications

Advanced assessment through intact glycopeptide analysis of Infliximab's biologics and biosimilar.

Kim Hyejin H   Bang Geul G   Park Ye Eun YE   Park Moonhee M   Choi Jung Hoon JH   Oh Myung Jin MJ   An Hyun Joo HJ   Yoo Jong Shin JS   Park Youngja Hwang YH   Kim Jin Young JY   Hwang Heeyoun H  

Frontiers in molecular biosciences 20221129


Characterization of therapeutic monoclonal antibodies (mAbs) represents a major challenge for analytical sciences due to their heterogeneity associated with post-translational modifications (PTMs). The protein glycosylation requires comprehensive identification, which could influence on the mAbs' structure and their function. Here, we demonstrated high-resolution tandem mass spectrometry with an ultra-high-performance liquid chromatography for characterization and comparison between biologics an  ...[more]

Similar Datasets

2022-08-03 | MSV000090073 | MassIVE
| S-EPMC9357431 | biostudies-literature
| S-EPMC7331092 | biostudies-literature
| S-EPMC5795011 | biostudies-literature
2024-02-21 | PXD038923 | Pride
| S-EPMC4599779 | biostudies-literature
| S-EPMC321528 | biostudies-literature
| S-EPMC5711575 | biostudies-literature
| S-EPMC9228347 | biostudies-literature
| S-EPMC9729625 | biostudies-literature