Single-cell microglial transcriptomics during demyelination defines a microglial state required for lytic carcass clearance.

ABSTRACT:

Background

Microglia regulate the response to injury and disease in the brain and spinal cord. In white matter diseases microglia may cause demyelination. However, how microglia respond and regulate demyelination is not fully understood.

Methods

To understand how microglia respond during demyelination, we fed mice cuprizone-a potent demyelinating agent-and assessed the dynamics of genetically fate-mapped microglia. We then used single-cell RNA sequencing to identify and track the microglial subpopulations that arise during demyelination. To understand how microglia contribute to the clearance of dead oligodendrocytes, we ablated microglia starting at the peak of cuprizone-induced cell death and used the viability dye acridine orange to monitor apoptotic and lytic cell morpho

SUBMITTER: Zia S 

PROVIDER: S-EPMC9746011 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature