CGRP-dependent sensitization of PKC-δ positive neurons in central amygdala mediates chronic migraine.

ABSTRACT:

Background

To investigate specific brain regions and neural circuits that are responsible for migraine chronification.

Methods

We established a mouse model of chronic migraine with intermittent injections of clinically-relevant dose of nitroglycerin (0.1 mg/kg for 9 days) and validated the model with cephalic and extracephalic mechanical sensitivity, calcitonin gene-related peptide (CGRP) expression in trigeminal ganglion, and responsiveness to sumatriptan or central CGRP blockade. We explored the neurons that were sensitized along with migraine chronification and investigated their roles on migraine phenotypes with chemogenetics.

Results

After repetitive nitroglycerin injections, mice displayed sustained supraorbital and hind paw mechanical hyperalgesia, which lasted

SUBMITTER: Chou TM 

PROVIDER: S-EPMC9746101 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature