Morphological and molecular characterization of the parasite Dipylidium caninum infecting an infant in Colombia: a case report.

ABSTRACT:

Background

Dipylidium caninum is the causal agent of dipylidiasis affecting mainly cats and dogs worldwide. Human cases of dipylidiasis are rare, and the diagnosis is prevalently based on morphological features of the parasite. Here we report the diagnosis of dipylidiasis through morphological and molecular characterization of D. caninum infecting an 11-month-old boy in Cajicá, Colombia.

Methods

Fresh faecal samples were obtained from the infant, and morphological identification of the parasite was performed through faecal smears. DNA was extracted from proglottids and used in PCR analyses for amplification of a 653-bp fragment of the nuclear ribosomal RNA (rRNA) encoding the 28S rRNA gene. A phylogeny study to better characterize the obtained DNA sequence was inferred using

SUBMITTER: Benitez-Bolivar P 

PROVIDER: S-EPMC9746177 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature