A high-efficiency differential expression method for cancer heterogeneity using large-scale single-cell RNA-sequencing data.

ABSTRACT: Colorectal cancer is a highly heterogeneous disease. Tumor heterogeneity limits the efficacy of cancer treatment. Single-cell RNA-sequencing technology (scRNA-seq) is a powerful tool for studying cancer heterogeneity at cellular resolution. The sparsity, heterogeneous diversity, and fast-growing scale of scRNA-seq data pose challenges to the flexibility, accuracy, and computing efficiency of the differential expression (DE) methods. We proposed HEART (high-efficiency and robust test), a statistical combination test that can detect DE genes with various sources of differences beyond mean expression changes. To validate the performance of HEART, we compared HEART and the other six popular DE methods on various simulation datasets with different settings by two simulation data generation mech

SUBMITTER: Yuan X 

PROVIDER: S-EPMC9746874 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature