Glucagon changes substrate preference in gluconeogenesis.

ABSTRACT: Fasting hyperglycemia in diabetes mellitus is caused by unregulated glucagon secretion that activates gluconeogenesis (GNG) and increases the use of pyruvate, lactate, amino acids, and glycerol. Studies of GNG in hepatocytes, however, tend to test a limited number of substrates at nonphysiologic concentrations. Therefore, we treated cultured primary hepatocytes with three identical substrate mixtures of pyruvate/lactate, glutamine, and glycerol at serum fasting concentrations, where a different U-¹³C- or 2-¹³C-labeled substrate was substituted in each mix. In the absence of glucagon stimulation, 80% of the glucose produced in primary hepatocytes incorporated either one or two ¹³C-labeled glycerol molecules in a 1:1 ratio, reflecting the high overall activit

SUBMITTER: Xu H 

PROVIDER: S-EPMC9747632 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature