Mechanisms of frustrated phagocytic spreading of human neutrophils on antibody-coated surfaces.

ABSTRACT: Complex motions of immune cells are an integral part of diapedesis, chemotaxis, phagocytosis, and other vital processes. To better understand how immune cells execute such motions, we present a detailed analysis of phagocytic spreading of human neutrophils on flat surfaces functionalized with different densities of immunoglobulin G (IgG) antibodies. We visualize the cell-substrate contact region at high resolution and without labels using reflection interference contrast microscopy and quantify how the area, shape, and position of the contact region evolves over time. We find that the likelihood of the cell commitment to spreading strongly depends on the surface density of IgG, but the rate at which the substrate-contact area of spreading cells increases does not. Validated by a theoretica

SUBMITTER: Francis EA 

PROVIDER: S-EPMC9748254 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature