Ameboid cell migration through regular arrays of micropillars under confinement.

ABSTRACT: Migrating cells often encounter a wide variety of topographic features-including the presence of obstacles-when navigating through crowded biological environments. Unraveling the impact of topography and crowding on the dynamics of cells is key to better understand many essential physiological processes such as the immune response. We study the impact of geometrical cues on ameboid migration of HL-60 cells differentiated into neutrophils. A microfluidic device is designed to track the cells in confining geometries between two parallel plates with distance h, in which identical micropillars are arranged in regular pillar forests with pillar spacing e. We observe that the cells are temporarily captured near pillars, with a mean contact time that is independent of h and e. By decreasing the v

SUBMITTER: Sadjadi Z 

PROVIDER: S-EPMC9748361 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature