A domino-like localized cascade toehold assembly amplification-based DNA nanowire for microRNA imaging in living cells.

ABSTRACT: High sensitivity and specificity imaging of miRNA in living cells plays an important role in understanding miRNA-related regulation and pathological research. Localized DNA circuits have shown good performance in reaction rate and sensitivity and have been proposed for sensitive imaging of miRNA in living cells. However, most reported localized DNA circuits have a high risk of derailment or a limited loading rate capacity, which hinder their further application. To solve these issues, we herein developed a domino-like localized cascade toehold assembly (LCTA) amplification-based DNA nanowire to achieve highly sensitive and highly specific imaging of miRNAs in living cells by using DNA nanowires as reactant delivery vehicles and confining both reactant probes in a compact space. The LCTA is

SUBMITTER: Yang Z 

PROVIDER: S-EPMC9749110 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature