Use of biosensors for rapid and sensitive detection of pesticides in food samples for food safety chemical risk assessment.

ABSTRACT: The utility of pesticides in the agricultural field is unquestionable, but at the same time pesticide use presents serious hazards to the environment and the human health. For that reason, detection of pesticides and their biotransformation products in food is of utmost importance. According to previous studies, esterase-based biosensors have been proposed as a viable and efficient solution for the detection of organophosphate pesticides. In this project, a double mutant of the thermostable esterase-2 (EST2) from Alicyclobacillus acidocaldarius was studied as a potential biosensor, for its ability to detect residual amounts of pesticides. Initial characterisation of the enzyme was performed, that included determination of optimal pH, thermophilicity, as well as kinetic analysis. Sub

SUBMITTER: Garefalaki V 

PROVIDER: S-EPMC9749373 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature