Gastric acid and escape to systemic circulation represent major bottlenecks to host infection by Citrobacter rodentium.

ABSTRACT: The gastrointestinal (GI) environment plays a critical role in shaping enteric infections. Host environmental factors create bottlenecks, restrictive events that reduce the genetic diversity of invading bacterial populations. However, the identity and impact of bottleneck events on bacterial infection are largely unknown. We used Citrobacter rodentium infection of mice, a model of human pathogenic Escherichia coli infections, to examine bacterial population dynamics and quantify bottlenecks to host colonization. Using Sequence Tag-based Analysis of Microbial Populations (STAMP) we characterized the founding population size (Nb') and relatedness of C. rodentium populations at relevant tissue sites during early- and peak-infection. We demonstrate that the GI environment severely restricts th

SUBMITTER: Woodward SE 

PROVIDER: S-EPMC9751147 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature