Chondrogenesis of Adipose-Derived Stem Cells Using an Arrayed Spheroid Format.

ABSTRACT:

Objective

The chondrogenic response of adipose-derived stem cells (ASCs) is often assessed using 3D micromass protocols that use upwards of hundreds of thousands of cells. Scaling these systems up for high-throughput testing is technically challenging and wasteful given the necessary cell numbers and reagent volumes. However, adopting microscale spheroid cultures for this purpose shows promise. Spheroid systems work with only thousands of cells and microliters of medium.

Methods

Molded agarose microwells were fabricated using 2% w/v molten agarose and then equilibrated in medium prior to introducing cells. ASCs were seeded at 50, 500, 5k cells/microwell; 5k, 50k, cells/well plate; and 50k and 250k cells/15 mL centrifuge tube to compare chondrogenic responses across spheroid

SUBMITTER: Gutierrez RA 

PROVIDER: S-EPMC9751248 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature