Role of Lymphatic Endothelium in Vascular Escape of Engineered Human Breast Microtumors.

ABSTRACT:

Introduction

Lymphatic vasculature provides a route for metastasis to secondary sites in the body. The role of the lymphatic endothelium in mediating the entry of breast cancer cells into the vasculature remains unclear.

Methods

In this study, we formed aggregates of MDA-MB-231 human breast carcinoma cells next to human microvascular lymphatic endothelial cell (LEC)-lined cavities in type I collagen gels to model breast microtumors and lymphatic vessels, respectively. We tracked invasion and escape of breast microtumors into engineered lymphatics or empty cavities under matched flow rates for up to sixteen days.

Results

After coming into contact with a lymphatic vessel, tumor cells escape by moving between the endothelium and the collagen wall, between endothelial cel

SUBMITTER: Seibel AJ 

PROVIDER: S-EPMC9751254 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature