Biochemical and genetic evidence for phospholipase C activity in Mycobacterium ulcerans.

ABSTRACT: This study reports the existence of phospholipase C and D enzymatic activities in Mycobacterium ulcerans cultures as determined by use of thin-layer chromatography to detect diglycerides in hydrolysates of radiolabeled phosphatidylcholine. M. ulcerans DNA sequences homologous to the genes encoding phospholipase C in Mycobacterium tuberculosis and Pseudomonas aeruginosa were identified by sequence analysis and DNA-DNA hybridization. Whether or not the phospholipase C and D enzymes of M. ulcerans plays a role in the pathogenesis of the disease needs further investigation.

SUBMITTER: Gomez A 

PROVIDER: S-EPMC97516 | biostudies-literature | 2000 May

REPOSITORIES: biostudies-literature