The Mutational Landscape of Cancer's Vulnerability to Ionizing Radiation.

ABSTRACT:

Purpose

Large-scale sequencing efforts have established that cancer-associated genetic alterations are highly diverse, posing a challenge to the identification of variants that regulate complex phenotypes like radiation sensitivity. The impact of the vast majority of rare or common genetic variants on the sensitivity of cancers to radiotherapy remains largely unknown.

Experimental design

We developed a scalable gene editing and irradiation platform to assess the role of categories of variants in cells. Variants were prioritized on the basis of genotype-phenotype associations from a previously completed large-scale cancer cell line radiation profiling study. Altogether, 488 alleles (396 unique single-nucleotide variants) from 92 genes were generated and profiled in an immorta

SUBMITTER: Gopal P 

PROVIDER: S-EPMC9751780 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature