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Characterization and Identification of a novel chromosome-encoded metallo-β-lactamase WUS-1 in Myroides albus P34.


ABSTRACT: In this study, we identified and characterized a novel chromosomally-encoded class B metallo-β-lactamase (MBL) gene designated bla WUS-1 in a carbapenem-resistant isolate Myroides albus P34 isolated from sewage discharged from an animal farm. Comparative analysis of the deduced amino acid sequence revealed that WUS-1 shares the highest amino acid similarities with the function-characterized MBLs MUS-1 (AAN63647.1; 70.73%) and TUS-1 (AAN63648.1; 70.32%). The recombinant carrying bla WUS-1 exhibited increased MICs levels against a number of β-lactam antimicrobials such as carbenicillin, ampicillin and imipenem, and β-lactamase inhibitors (clavulanic acid and tazobactam). The metallo-β-lactamase WUS-1 could also hydrolyze these antimicrobials and the hydrolytic activities could be inhibited by EDTA. Genetic context analysis of bla WUS-1 revealed that no mobile genetic element was found in its surrounding region. The plasmid pMA84474 of Myroides albus P34 harbored 6 resistance genes (bla OXA-347, aadS, bla MYO-1, ereD, sul2 and ermF) within an approximately 17 kb multidrug resistance (MDR) region. These genes, however, were all related to mobile genetic elements.

SUBMITTER: Liu S 

PROVIDER: S-EPMC9751785 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Characterization and Identification of a novel chromosome-encoded metallo-β-lactamase WUS-1 in <i>Myroides albus</i> P34.

Liu Shuang S   Zhang Lei L   Feng Chunlin C   Zhu Jin J   Li Anqi A   Zhao Jingxuan J   Zhang Yuan Y   Gao Mengdi M   Shi Weina W   Li Qiaoling Q   Zhang Xueya X   Zhang Hailin H   Xu Teng T   Lu Junwan J   Bao Qiyu Q  

Frontiers in microbiology 20221201


In this study, we identified and characterized a novel chromosomally-encoded class B metallo-β-lactamase (MBL) gene designated <i>bla</i> <sub>WUS-1</sub> in a carbapenem-resistant isolate <i>Myroides albus</i> P34 isolated from sewage discharged from an animal farm. Comparative analysis of the deduced amino acid sequence revealed that WUS-1 shares the highest amino acid similarities with the function-characterized MBLs MUS-1 (AAN63647.1; 70.73%) and TUS-1 (AAN63648.1; 70.32%). The recombinant c  ...[more]

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