1208. Polymorphic Locus SerMT1 for Sequence-based Typing of Serratia marcescens

ABSTRACT: Abstract

Background

Outbreaks of pathogenic agents within healthcare facilities may have sporadic or nosocomial sources. Effective intervention requires distinguishing between these sources, which in turn requires epidemiological investigation in conjunction with typing of pathogen isolates. For the latter, established methods range from ribotyping and pulse-field gel electrophoresis to multilocus sequence typing and whole genome sequencing. Due to technical complexities and costs associated with these methods, however, typing is rarely pursued. Polymorphic locus sequence typing (PLST) addresses these issue by employing conventional PCR and Sanger sequencing; the key to PLST is its focus on a tandem repeat-containing locus exhibiting maximal variation due to combinations of single

SUBMITTER:  

PROVIDER: S-EPMC9752729 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature