Dynamic feedback regulation for efficient membrane protein production using a small RNA-based genetic circuit in Escherichia coli.

ABSTRACT:

Background

Membrane proteins (MPs) are an important class of molecules with a wide array of cellular functions and are part of many metabolic pathways. Despite their great potential-as therapeutic drug targets or in microbial cell factory optimization-many challenges remain for efficient and functional expression in a host such as Escherichia coli.

Results

A dynamically regulated small RNA-based circuit was developed to counter membrane stress caused by overexpression of different MPs. The best performing small RNAs were able to enhance the maximum specific growth rate with 123%. On culture level, the total MP production was increased two-to three-fold compared to a system without dynamic control. This strategy not only improved cell growth and production of the studied MPs,

SUBMITTER: Guidi C 

PROVIDER: S-EPMC9753035 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature