Acetyltransferase NAT10 regulates the Wnt/β-catenin signaling pathway to promote colorectal cancer progression via ac⁴C acetylation of KIF23 mRNA.

ABSTRACT:

Background

N⁴-acetylcytidine (ac⁴C) as a significant RNA modification has been reported to maintain the stability of mRNA and to regulate the translation process. However, the roles of both ac⁴C and its 'writer' protein N-acetyltransferase 10 (NAT10) played in the disease especially colorectal cancer (CRC) are unclear. At this point, we discover the underlying mechanism of NAT10 modulating the progression of CRC via mRNA ac⁴C modification.

Methods

The clinical significance of NAT10 was explored based on the TCGA and GEO data sets and the 80 CRC patients cohort of our hospital. qRT-PCR, dot blot, WB, and IHC were performed to detect the level of NAT10 and ac⁴C modification in CRC tissues and matched adjacent tissues. CCK-

SUBMITTER: Jin C 

PROVIDER: S-EPMC9753290 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature