Automated assembly scaffolding using RagTag elevates a new tomato system for high-throughput genome editing.
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ABSTRACT:
SUBMITTER: Alonge M
PROVIDER: S-EPMC9753292 | biostudies-literature | 2022 Dec
REPOSITORIES: biostudies-literature
Publications
Automated assembly scaffolding using RagTag elevates a new tomato system for high-throughput genome editing.
Alonge Michael M Lebeigle Ludivine L Kirsche Melanie M Jenike Katie K Ou Shujun S Aganezov Sergey S Wang Xingang X Lippman Zachary B ZB Schatz Michael C MC Soyk Sebastian S
Genome biology 20221215 1
Advancing crop genomics requires efficient genetic systems enabled by high-quality personalized genome assemblies. Here, we introduce RagTag, a toolset for automating assembly scaffolding and patching, and we establish chromosome-scale reference genomes for the widely used tomato genotype M82 along with Sweet-100, a new rapid-cycling genotype that we developed to accelerate functional genomics and genome editing in tomato. This work outlines strategies to rapidly expand genetic systems and genom ...[more]
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