Comparison of structural variants detected by PacBio-CLR and ONT sequencing in pear.

ABSTRACT:

Background

Structural variations (SVs) have recently become a topic of great interest in the area of genetic diversity and trait regulation. As genomic sequencing technologies have rapidly advanced, longer reads have been used to identify SVs at high resolution and with increased accuracy. It is important to choose a suitable sequencing platform and appropriate sequencing depth for SV detection in the pear genome.

Results

In this study, two types of long reads from sequencing platforms, continuous long reads from Pacific Biosciences (PB-CLR) and long reads from Oxford Nanopore Technologies (ONT), were used to comprehensively analyze and compare SVs in the pear genome. The mapping rate of long reads was higher when the program Minimap2 rather than the other three mapping tool

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9753399 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature